Coding

Part:BBa_K3001002

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-14)


Cas13a Lbu Coding Sequence

This protein is an RNA cleaving enzyme called Cas13a that was isolated from Leptotrichia buccalis (Lbu). It interacts with a direct repeat stem loop on a CRISPR RNA (crRNA) that contains a specific sequence for a target RNA transcript. This construct is designed to be able to be expressed under an inducible promoter (T7). The protein itself has several purification tags (His, Twin strep tag, and a SUMO tag). This construct was ordered from Addgene. Please see part BBa_K3001003 for experience using this part.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 349
    Illegal BglII site found at 1285
    Illegal BglII site found at 1495
    Illegal BglII site found at 1549
    Illegal BglII site found at 1780
    Illegal BglII site found at 2053
    Illegal BglII site found at 2539
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1068
    Illegal PstI site found at 1923
    Illegal PstI site found at 2862
    Illegal NgoMIV site found at 2001
    Illegal NgoMIV site found at 2637
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None